Anaerobic soil disinfestation (ASD) is extensively used to regulate soilborne illnesses in natural crop manufacturing. The impact of ASD used completely different sealed movies on soilborne pathogens and strawberry progress was evaluated in two laboratory research and two area trials. Underneath maltose as carbon sources, 28 °C temperature and 30% of soil moisture optimum situations ASD decreased Fusarium spp. and Phytophthora spp. by 100%. ASD used maltose as an natural modification and sealed with completely impermeable movie (TIF) obtained the best suppression (>96%) towards Fusarium spp. and Phytophthora spp. (>91%). In response to the laboratory outcomes, the efficacy of ASD using 6 or 9 t/ha maltose and sealing with TIF was evaluated and in contrast with reference therapy with chloropicrin (Pic) or solarization (Sol) within the area trials. In contrast with the untreated soil, ASD therapies significantly diminished the pathogenic inhabitants of Fusarium spp. and Phytophthora spp., and efficiently managed the harm of fusarium wilt with proof of decrease mortality (6%). ASD considerably elevated soil diet promoted plant progress and elevated strawberry yield, which was comparable because the Pic, however higher than Sol therapy. The analyzed fungal and bacterial microbiota didn’t present important variations within the taxonomic richness and variety between the in contrast therapies. Nonetheless, the abundance of some bacterial and fungal taxa tended to vary between handled. The proof confirmed that including maltose and sealing TIF for ASD has the potential to interchange Pic for pathogen management in industrial strawberry manufacturing.